Kanamycin in Cereal Biotechnology: A Screening or a Selectable Marker?

Kanamycin is a widely used selection 53-264817 agent in dicot-plant genetic transformation systems.In monocots, however, it does not seem to be effective as it has no or minimal effect on the normal growth of non-transformed plants.Kanamycin was previously demonstrated to bleach the ssp218dda-sf pigments of the non-transgenic plants.

This may yield the idea that kanamycin can be used as an effective screening marker rather than a selectable marker in monocots.Copyright (c) 2021 Malik Nawaz Shuja, Hasan Riaz, Muhsin Jamal, Muhammad Imran.

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